06 19월_IRP:OT

Info장소:Neurogenetics Lab(NGLLab), PBC 1F room 108, office:PBC 1F room#(백· 쓴 테 교수)일시:2023.06.19.2023.08.31(예정)기록 2:00교수 office로 OTw/선영 2:30연구실들에게 인사, 사수에게 Concept안내 받다 오늘 숙제 1.Cloning공부 2. Mini-preprep공부 3. Rheb논문을 읽는 4논문을 발표한다.PI3K-AKT-mTOR pathway공부전반적인 ConceptGene mutation(pCIG-Rheb Y35L)→ Cloning→ Midi-prep→세포주 도입(w/lipofectamine&transposase)(or by heat shock, electronic shock)→ FACs sorting→ Western blot(Gain of Function돌연변이것으로)(→ MEA, dysmorphic neuron, dyslamination)→ Cell migration checkRheb project/Paper 1pCIG-Rheb WT→ Mutagenesis(pCIG-Rheb Y35L)→ DD-Rheb(WT&Y35L), sub-cloningDD(Destabilizing Domain)system for in vitro/Paper 2=DD system을이용한 단백질발현 조절Info 장소: Neurogenetics Lab(NGLLab), PBC 1F room 108, office: PBC 1F room #(백승태 교수) 일시: 2023.06.19.~2023.08.31 (예정) 기록 2:00 교수 office로 OTw/성영 2:30 연구실 분들께 인사드리고 사수님께 Concept 안내받는 오늘의 숙제 1. Cloning 공부 2. Mini-prep 공부 3. Rheb 논문 읽기 4개 논문 발표하기. PI3K-AKT-mTOR pathway 공부전반적인 ConceptGene mutation (pCIG-Rheb Y35L)→ Cloning→ Midi-prep→ 세포주 도입 (w/ lipofectamine & transposase) (or by heat shock, electronic shock)→ FACs sorting→ Western blot (Gain of Function 돌연변이 확인)(→ MEA, dysmorphic neuron、 dyslamination)→ Cell migration checkRheb project / Paper 1pCIG-Rheb WT→ Mutagenesis (pCIG-Rheb Y35L)→ DD-Rheb (WT & Y35L), sub-cloningDD (Destabilizing Domain) system for in vitro / Paper 2= DD system을 이용한 단백질 발현 조절